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Quantitative Proteomics: RSC [Hardcover]

Item : Quantitative Proteomics: RSC [Hardcover]
Price : $250.99 (see below)
Category : Books
ISBN-10 ISBN-10 : 1849738084
ISBN-13 ISBN-13 : 9781849738088
Publisher Publisher : Royal Society of Chemistry
Pages Pages : 390
Binding Binding : Hardcover
Item ID : 100105673

As a component of post-genome science, the field of proteomics has assumed great prominence in recent years. Whereas quantitative analyses dealt at first with relative quantification, a greater emphasis is now placed on absolute quantification and consideration of proteome dynamics. Coverage of the topic of quantitative proteomics requires consideration both of the analytical fundamentals of quantitative mass spectrometry and the specific demands of proteome analysis. This exemplary book is essential reading for analytical and biological spectroscopists working in proteomic research.

As a component of post-genome science, the field of proteomics has assumed great prominence in recent years. Whereas quantitative analyses focussed initially on relative quantification, a greater emphasis is now placed on absolute quantification and consideration of proteome dynamics. Coverage of the topic of quantitative proteomics requires consideration both of the analytical fundamentals of quantitative mass spectrometry and the specific demands of the problem being addressed.

Quantitative Proteomics aims to outline the state of the art in mass spectrometry-based quantitative proteomics, describing recent advances and current limitations in the instrumentation used, together with the various methods employed for generating high quality data. Details on both strategies describing how stable isotope labelling can be applied and methods for performing quantitative analysis of proteins in a label-free manner are given. The utility of these strategies to understanding cellular protein dynamics are then exemplified with chapters looking at spatial proteomics, dynamics of protein function as determined by quantifying changes in protein post-translational modification and protein turnover. Finally, a key application of these techniques to biomarker discovery and validation is presented, together with the rapidly developing area of quantitative analysis of protein-based foodstuffs.

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