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DNA Conjugates and Sensors: RSC [Hardcover]

Item : DNA Conjugates and Sensors: RSC [Hardcover]
Price : $234.99 (see below)
Category : Books
ISBN-10 ISBN-10 : 1849734275
ISBN-13 ISBN-13 : 9781849734271
Publisher Publisher : Royal Society of Chemistry
Pages Pages : 316
Binding Binding : Hardcover
Item ID : 100060745

Applications of nucleic acids have developed recently to provide solutions for biosensors, diagnostic tools and as platforms for the assembly of complex structures. These developments have been possible as their base sequence can be used to assemble precise structures following simple and predictable rules. Self-assembled DNA can then be amplified using polymerase chain reaction (PCR) and this ultimately enables the preparation of synthetic nucleic acids. Their use as molecular tools or DNA-conjugates has recently been enhanced by the addition of other groups including enzymes, fluorophores and small molecules. Written by leaders in the field, this volume describes the preparation and application of these DNA-conjugates. Several have been used as sensors (aptamers, riboswitches and nanostructures) based on the ability of nucleic acids to adopt specific structures in the presence of ligands, whilst others link reporter groups such as proteins or fluorophores to RNA or DNA for detection, single molecule studies, and increasing the sensitivity of PCR. The book is relevant to researchers in areas related to analytical chemistry, chemical biology, medicinal chemistry, molecular pharmacology, and structural and molecular biology.The preparation of synthetic nucleic acids has been possible for several years, but their use as molecular tools has recently been enhanced by the addition of other groups. These include enzymes, fluorophores and small molecules. Modification to the DNA bases or backbone can also improve biological stability and increase sensitivity. Written by leaders in the field, this volume describes the preparation and application of these DNA-conjugates. Several have been used as biological sensors (aptamers, riboswitches and nanostructures). Others link reporter groups such as proteins or fluorophores to RNA or DNA for detection, single molecule studies, and increasing the sensitivity of PCR. The book will interest researchers in related areas of chemistry,lÉ*

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