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Cell Reprogramming: Methods and Protocols [Mixed media product]

Item : Cell Reprogramming: Methods and Protocols [Mixed media product]
Price : $206.99 (see below)
Category : Books
ISBN-10 ISBN-10 : 1493928473
ISBN-13 ISBN-13 : 9781493928477
Publisher Publisher : Humana Press
Binding Binding : Mixed media product
Item ID : 100055777

This volume provides an understanding of the factors involved in nuclear reprogramming, which is essential for the success of reprogramming. The book is aimed at reprogramming differentiated cells and germ line transmission of pluripotent stem cells and features chapters that deal with reprogramming-related issues such as analysis of mitochondrial DNA in reprogrammed cells and the isolation of reprogramming intermediates; alternative methods for nuclear transfer; the production of germ-line chimeras from embryonic stem cells and induced pluripotent stem cells; and neonatal care and management of somatic cell nuclear transfer derived offspring.?Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, is the perfect guide for molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians and animal care technicians involved with reprogramming, nuclear transfer and transgenesis.

Part I Background

1. Cellular Reprogramming in Basic and Applied Biomedicine: The Dawn of Regenerative Medicine
Wendy Dean

Part II De Novo Reprogramming

2.?Synthetic mRNA Reprogramming of Human Fibroblast Cells
Jun Liu and Paul J. Verma

3.?MicroRNA Mediated Reprogramming of Somatic Cells into Induced Pluripotent Stem Cells
Shelley E. Swing and Bhanu Prakash V.L. Telugu

4.?Generation of Footprint-Free Induced Pluripotent Stem Cells from lÓ

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